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ICLE 2022 | Cellular engineering using nanoparticle-sensitized photoporation

Chimeric antigen receptor (CAR)-T cell therapies are commonly generated using viral transduction methods, however, given high manufacturing costs and safety concerns, there is ongoing research into alternative transfection technologies that could be utilized in ex vivo cell manipulation. Dominika Berdecka, University of Ghent, Ghent, Belgium, describes nanoparticle-sensitized photoporation technology, which involves incubating cells with photoresponsive nanoparticles followed by laser irradiation, leading to transient membrane permeabilization. This technology offers the ability to combine high delivery efficiency with reduced cytotoxicity, can facilitate the delivery of a range of different compounds to a broad range of cell types and its clinical potential is currently under investigation. This interview took place at the International Conference on Lymphocyte Engineering (ICLE) 2022.