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ISAL 2025 | Studying AML in mouse models: methods and challenges
Christina Mayerhofer, MD, Harvard University, Cambridge, MA, discusses the methods and challenges of studying acute myeloid leukemia (AML) in mouse models. She highlights two methods: using irradiation to engraft murine AML and patient-derived xenograft (PDX) models. Dr Mayerhofer emphasizes the importance of considering the potential side effects of chemotherapy agents, such as doxorubicin, when designing mouse models. This interview took place at the 19th International Symposium on Acute Leukemias (ISAL XIX) in Munich, Germany.
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Transcript
Usually, there are two ways to do that in the mouse models. There is the treatment of mouse leukemia, which usually requires irradiation to engraft murine AML, usually driven by MLL AF9 in the mice. And this irradiation can, of course, affect also other cells. So we use a mouse model, preferably, that does not require irradiation, that requires no preconditioning. So we can also study other cell types around that...
Usually, there are two ways to do that in the mouse models. There is the treatment of mouse leukemia, which usually requires irradiation to engraft murine AML, usually driven by MLL AF9 in the mice. And this irradiation can, of course, affect also other cells. So we use a mouse model, preferably, that does not require irradiation, that requires no preconditioning. So we can also study other cell types around that. The other option is to use a patient-derived xenograft PDX model. These models are much more sensitive. And so you might want to reduce chemotherapy doses or whichever chemotherapy regimen you’re using. Also, it’s important to keep in mind that, for instance, doxorubicin can severely hurt the livers in these strains. So you might want to think about altering your delivery methods in mice.
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